Hybrid Tomato Variety &#39;H2012&#39;

ABSTRACT

Hybrid tomato variety ‘H2012’ is described. The tomato variety is a ground-culture hybrid processing tomato variety suitable for machine harvest and is adaptable to the arid climactic conditions of regions such as California, USA, and Extremadura, Spain. It is a main season variety with resistance to  verticillium  wilt race 1,  fusarium  wilt races 1, 2, and 3, root knot nematode, and tomato spotted wilt virus. While the high viscosity of its processed juice make fruit of ‘H2012’ particularly valuable for production of sauces and paste, its uniform and attractive fruit are also valuable for whole-pack and peel/dice uses. The combination of extended field storage of the fruit with its resistance to  fusarium  wilt race 3 and tomato spotted wilt virus make it valuable to the tomato processing industry.

FIELD

This disclosure relates to the field of plant breeding. In particular,this disclosure relates to a new variety of tomato, Solanum lycopersicumvariety denominated ‘H2012’.

BACKGROUND

Breeding improved tomato varieties involves providing genetics that givean advantage to the grower, processor, consumer, or other members of thesupply chain. The improvement may be in the form of field performance,disease resistance, factory performance, or a fruit qualitycharacteristic. For a tomato variety to be suitable to be grown forprocessing, the variety must have a concentrated fruit setting andmaturity, firm fruit, and sufficient rot tolerance to allow early fruitto remain rot-free while later fruit continues to develop and ripen.

Most tomato varieties for commercial processing are hybrids resultingfrom a cross pollination of two true-breeding, inbred parents. Throughthe use of true-breeding lines, a hybrid is produced that often displayscharacteristics of each parent, and often demonstrates characteristicsthat are superior to either parent alone, or that allow a hybrid to maskinadequacies of the individual parents.

Processing tomato varieties combining resistance to verticillium wiltrace 1 (Verticillium dahlia), fusarium wilt races 1,2 (Fusariumoxysporum pv lycopersici), and root knot nematode (Meloidogyneincognita) are highly desirable in most climates around the world.Moreover, in regions such as California, the industry has seen aconsiderable increase in pressure from tomato spotted wilt virus (TSWV)and a third race of fusarium wilt race 3. Thus, varieties withresistance to these pathogens are in high demand by both growers andprocessors to ensure a productive crop cycle.

Processing tomato quality parameters differ from those of fruit used inthe fresh market. The processing characteristics are typicallydetermined using a sample of hot-break tomato pulp or juice produced ina consistent manner to those familiar with the art. For example, a fixedmass of tomatoes may be cooked in a microwave oven for several minutesto halt any enzymatic breakdown of the sample, lost water is replaced,and the sample is pulped to remove skins and seed to produce a uniformjuice sample. The juice sample can be analyzed for various qualityparameters important to processing tomato including but not limited togross viscosity measures such as juice Bostwick, soluble solids measuresusing a refractometer (°Brix), measures of acidity and pH, and measuresof color (e.g., a Hunter a/b score). The value of these traits dependson the product that is being commercially produced by the processingfactory. In some instances, a factory will put a higher value on a thickviscosity variety, whereas in other instances, a thin viscosity willmake a superior product and is preferred.

SUMMARY

Provided herein is a new and distinct tomato variety named ‘H2012’ thatproduces high yields of medium, uniform, smooth fruit and is adapted toarid climates such as California, and arid regions of Europe such asSpain. The variety ‘H2012’ has resistance to verticillium wilt race 1,three known races of fusarium wilt, root knot nematode, and tomatospotted wilt virus. The fruit of ‘H2012’ are extremely firm and haveexcellent red color inside and out with an average fruit weight of 73grams. ‘H2012’ presents an advantage over industry standard varietiessuch as ‘H5608’ in that it has demonstrated resistance to fusarium wiltrace 3 which has become a significant production limitation in the stateof California. The tolerance to fruit rot in ‘H2012’ is very good,allowing for once-over machine harvesting applications especially whenextended ripe-fruit field holding is desired.

In one aspect, the present disclosure is directed to tomato seeddesignated as ‘H2012’ having ATCC Accession Number PTA-126914. In oneaspect, the present disclosure is directed to a tomato plant, as well asany plant part or portion isolated therefrom, produced by growing‘H2012’ tomato seed. In another aspect, the present disclosure isdirected to a tomato plant or part isolated therefrom having all thephysiological, morphological, and/or genetic characteristics of a tomatoplant produced by growing ‘H2012’ tomato seed having ATCC AccessionNumber PTA-126914. In yet another aspect, the present disclosure isdirected to a tomato plant having all of the physiological andmorphological characteristics of tomato variety ‘H2012’, andspecifically including those listed in Table 1 below, whereinrepresentative seed is deposited under ATCC Accession Number PTA-126914.

In still another aspect, the present disclosure is directed to tomatoseed having at least a first set of the chromosomes of tomato variety‘H2012’, wherein representative seed is deposited under ATCC AccessionNumber PTA-126914. In another aspect, the present disclosure is directedto an F₁ hybrid tomato seed, methods of making F₁ hybrid tomato seed,plants grown from the seed, leaf, ovule, pollen, rootstock, scion,fruit, cotyledon, meristem, anther, root, root tip, pistil, flower,stem, calli, stalk, hypocotyl, pericarp, or portion thereof isolatedtherefrom having ‘H2012’ as a parent, wherein ‘H2012’ is grown from‘H2012’ tomato seed having ATCC Accession Number PTA-126914. Thedisclosure is also directed to a method of producing a tomato plantderived from tomato variety ‘H2012’, including crossing a plant oftomato variety ‘H2012’ with another tomato plant. The method may furthercomprise harvesting seed from the F₁ hybrid tomato seed and/or crossingthe F₁ hybrid tomato plant with itself or another plant to produce seedfrom a progeny plant.

Tomato plant parts include leaf, ovule, pollen, rootstock, scion, fruit,cotyledon, meristem, anther, root, root tip, pistil, flower, stem,calli, stalk, hypocotyl, pericarp, the like, and any portion thereof. Inanother embodiment, the present disclosure is further directed to tomatofruit, stem, leaf, root, root tip, pollen, rootstock, scion, ovule,seed, and flower, and any portion thereof, isolated from ‘H2012’ tomatoplants. In one aspect, the plant part comprises at least one cell fromtomato variety ‘H2012’. In another aspect, the present disclosure isfurther directed to tissue culture of regenerable cells derived from‘H2012’ tomato plants. In one aspect, the tissue culture of regenerablecells has all the physiological and morphological characteristics oftomato variety ‘H2012’. The disclosure is further directed to a tomatoplant regenerated from tissue culture. In another aspect, the disclosureis directed to a protoplast produced from tissue culture and a plantregenerated from the protoplast. At least in some approaches, the plantregenerated from the tissue culture or protoplast has all of thephysiological and morphological characteristics of tomato variety‘H2012’, and specifically including those listed in Table 1.

In another aspect, the disclosure is directed to a method for producinga plant part, which at least in one aspect is tomato fruit, andharvesting the plant part. In another aspect, the plant part comprisesat least one cell from tomato variety ‘H2012’.

The disclosure also is directed to vegetatively propagating a plant oftomato variety ‘H2012’ by obtaining a part of the plant and regeneratinga plant from the plant part. At least in some approaches, theregenerated plant has all of the physiological and morphologicalcharacteristics of tomato variety ‘H2012’, and specifically includingthose listed in Table 1.

In yet another aspect, the present disclosure is further directed to amethod of selecting tomato plants by a) growing ‘H2012’ tomato plantswherein the ‘H2012’ plants are grown from tomato seed having ATCCAccession Number PTA-126914; and b) selecting a plant from step a). Inanother aspect, the present disclosure is further directed to tomatoplants, plant parts, and seeds produced by the tomato plants, where thetomato plants are isolated by the selection method.

In another aspect, the present disclosure is further directed to amethod of breeding tomato plants by crossing a tomato plant with a plantgrown from ‘H2012’ tomato seed having ATCC Accession Number PTA-126914.In another aspect, the tomato plant of tomato variety ‘H2012’ isself-pollinated. In still another aspect, the present disclosure isfurther directed to tomato plants, tomato parts from the tomato plants,and seeds produced therefrom where the tomato plant is isolated by thebreeding method.

In another aspect, the disclosure relates to a plant of tomato variety‘H2012’ comprising a transgene and/or a single locus conversion, and anyseeds or plant parts isolated therefrom. The disclosure also relates tomethods for preparing a plant of tomato variety ‘H2012’ comprising atransgene and/or a single locus conversion. In one aspect, the plantcomprising a single locus conversion and/or transgene has all thephysiological and morphological characteristics of tomato variety‘H2012’.

BRIEF DESCRIPTION OF THE DRAWING

The patent or application file contains at least one drawing executed incolor. Copies of this patent or patent application publication withcolor drawings will be provided by the office upon request and paymentof the necessary fee.

The Figure illustrates the fruit and plant of ‘H2012’.

DETAILED DESCRIPTION

Described herein is the new and distinct tomato variety named ‘H2012’that was developed to provide a ground-culture hybrid tomato variety(i.e., not grown on stakes) that is suitable for machine harvest and isadaptable to the climactic conditions of regions such as California,USA.

Processing tomato varieties combining resistance to verticillium wiltrace 1 (Verticillium dahlia), fusarium wilt race 1, 2 and 3 (Fusariumoxysporum pv. lycopersici), root knot nematode (Meloidogyne incognita),and tomato spotted wilt virus are highly desirable in many tomatoproducing climates around the world. Varieties for processing are alsovalued when they are very firm and have a maturity concentrated enoughto allow for machine harvesting. Varieties with strong tolerance to ripefruit rots (extended field storage) are also desirable because they areconducive to once-over machine harvesting, particularly in regions whererain can occur during harvest or when there are delays in a harvestingschedule.

Tomato plants of ‘H2012’ are resistant to verticillium wilt race 1,three known races of fusarium wilt, root knot nematode, and tomatospotted wilt virus. Medium-sized plants are concentrated and bushy withgood vine cover and health Foliage is medium green in color withsubstantial leaf roll. Fruit of ‘H2012’ are very firm, and medium insize (73 gram). Both internal and external color of the fruit is deepred. Maturity for once-over machine harvest is considered in the mainseason class, similar to an industry standard ‘H5608’ and distinctlyearlier than another standard ‘Nunhems 6428’; as with most varietiesactual days to maturity can vary based upon environmental conditions.Fruit are also considered to have extended field storage, which delaysthe onset of fruit rots after reaching red ripe maturity.

Stability of Variety ‘H2012’

The variety is uniform and stable within commercially acceptable limits.As is true with other tomato varieties, a small percentage of variantscan occur within commercially acceptable limits for almost anycharacteristic during the course of repeated multiplication. However, novariants were observed during the three years in which the variety wasobserved to be uniform and stable.

TABLE 1 Characterization of tomato variety ‘H2012’ compared to twoprocessing industry standards, ‘H5608’ and ‘H3402’ Character ‘H2012’‘H5608’ ‘H3402’ Seedling Anthocyanin in hypocotyl Present PresentPresent Habit of 3-4 Wk seedling Normal Normal Normal Mature PlantHeight (cm) 106 cm 111 cm 109 cm Growth Type Determinate DeterminateDeterminate Form Normal Sprawling Normal Size of Canopy Medium LargeMedium Habit Sprawling Sprawling Semi-erect Stem Branching ProfuseProfuse Profuse Branching at Cotyledon Absent Absent Absent # nodesbelow first 1-4 nodes 4-7 nodes 7-10 nodes inflorescence # nodes betweenearly 1-2 nodes 1-2 nodes 1-2 nodes inflorescence (1^(st)-2^(nd),2^(nd)-3^(rd)) # nodes between later 1-2 nodes 1-2 nodes 1-2 nodesinflorescence Pubescence on younger stems Moderate Sparse Moderate LeafType Tomato Tomato Tomato Morphology Compound with Compound withCompound with major and minor major and minor major and minor leaflets(image 2) leaflets (image 2) leaflets (image 2) Margins of MajorLeaflets Shallow Nearly entire Shallow toothed Marginal Rolling orWiltiness Moderate Moderate Absent Onset of Leaflet Rolling MidseasonMidseason Midseason Surface of Major Leaflets Rugose Rugose RugosePubescence Smooth Normal Sparse Leaf Vein Color Transparent TransparentTransparent Inflorescence Type Simple Forked Simple # flowers ininflorescence 6 5 7 average Leafy or “running” Absent Absent Absentinflorescence Flower Calyx Normal Normal Normal Calyx-Lobes Shorter thanShorter than Shorter than corolla corolla corolla Corolla Color Old goldYellow Yellow Style pubescence Absent Sparse Sparse Anthers Fused/TubedFused/Tubed Fused/Tubed Fasciation Absent Absent Absent Fruit Typicalshape Blocky Oval Blocky Oval Blocky Oval in longitudinal section Shapeof transverse section Round Round Round Shape of stem end Flat IndentedFlat Shape of blossom end Nippled Flat Indented Shape of pistil scar DotDot Dot Abscission layer Absent Absent Absent point of detachment Atcalyx At calyx At calyx fruit at harvest Length of pedicel (joint to 0.7cm 0.9 cm 1.1 cm calyx attachment) (cm) Length of mature fruit 6.0 5.25.8 (stem axis) (cm) Diameter of fruit at widest 3.8 3.8 4.5 point (cm)Weight of Mature Fruit (g) 73 73 62 Number of Locules 2 2-4 2 FruitSurface Smooth Smooth Smooth Fruit Base Color Light Green Yellow GreenApple/medium (Mature Green Stage) green Fruit Pattern (mature greenUniform Uniform Uniform stage) Shoulder color if different n/a n/a n/afrom base Fruit color full ripe Red Red Red Flesh color full ripe RedRed Red Flesh color Uniform Uniform Uniform Locular gel color oftable-ripe Red Yellow Red fruit Ripening Uniform Uniform Uniform StemScar Size Small Small Small Core Coreless Coreless Coreless EpidermisColor Yellow Yellow Yellow Epidermis Normal Normal Normal EpidermisTexture Average Tough Average Thickness or Pericarp (mm) 7.0 8.0 8.5Resistance to Fruit Disorder Not tested Not tested Not tested Diseaseand Pest Reactions Viral Diseases Cucumber Mosaic n/t n/t n/t Curly Topn/t n/t n/t Potato-y Virus n/t n/t n/t Blotch Ripening n/t n/t n/tTobacco Mosaic Race 0 n/t n/t n/t Tobacco Mosaic Race 1 n/t n/t n/tTobacco Mosaic Race 2 n/t n/t n/t Cracking, Concentric n/t n/t n/tTobacco Mosaic Race 2² n/t n/t n/t Tomato Spotted Wilt ResistantResistant Susceptible Tomato Yellows n/t n/t n/t Gold Fleck n/t n/t n/tOthers n/t n/t n/t Bacterial Disease Bacterial Canker Min. resistantSusceptible Min. Resistant (Clavibacter michiganense) Bacterial Soft Rot(Erwinia n/t n/t n/t carotovora) Bacteria Speck race 0 SusceptibleResistant Resistant (Pseudomonas tomato) Bacterial Spot (XanthomonasMin. Resistant Susceptible Susceptible spp) Bacterial Wilt n/t n/tSusceptible (Ralstonia solanacearum) Other Bacterial Disease n/t n/t n/tFungal Disease Anthracnose (Colletotrichum n/t n/t Susceptible spp.)Brown Root Rot or Corky n/t n/t Susceptible Root (Pyrenochaetalycopersici) Collar Rot or Stem Canker n/t Susceptible Susceptible(Alternaria solani) Early Blight Defoliation Susceptible SusceptibleMin. Resistant (Alternaria solani) Fusarium Wilt Race 1 ResistantResistant Resistant (F. oxysporum f. lycopersici) Fusarium Wilt Race 2Resistant Resistant Resistant (F. oxysporum f. lycopersici) FusariumWilt Race 3 Resistant Susceptible Susceptible (F. oxysporum f.lycopersici) Grey Eeaf Spot (Stemphylium n/t n/t n/t spp.) Fate Blight,race 0 n/t n/t Susceptible (Phytophtliora infestans) Late Blight, race 1n/t n/t n/t Leaf Mold race 1 n/t n/t n/t (Cladosporium fulvum) Leaf Moldrace 2 n/t n/t n/t (Cladosporium fulvum) Leaf Mold race 3 n/t n/t n/t(Cladosporium fulvum) Leaf Mold Other Races: n/t n/t n/t Nail head Spot(Alternaria n/t n/t n/t tomato) Septoria Leaf spot (S. n/t n/t n/tLycopersici) Target Leaf spot (Corynespora n/t n/t n/t cassiicola)Verticillium Wilt Race 1 Resistant Resistant Resistant (V. dahliaerace 1) Verticillium Wilt Race 2 n/t n/t n/t (V. dahliae race 2) OtherFungal Disease n/t n/t n/t Insects and Pests Colorado Potato Beetle n/tn/t n/t (L. decemlineata) Root Knot Nematode (M. sp.) ResistantResistant Resistant Spider Mites (Tetranychus n/t n/t n/t spp.) SugarBeet Army Worm (S. n/t n/t n/t exigua) Tobacco Flea Beetle (E. n/t n/tn/t hiritipennis) Tomato Hornworm n/t n/t n/t (M. quinquemaculata)Tomato Fruit worm (H. zea) n/t n/t n/t Whitefly (T. vaporariorum) n/tn/t n/t Other n/t n/t n/t

Chemistry and Composition of Full-Ripe Fruits

TABLE 2 Hot-break tomato juice characteristics for new variety ‘H2012’and two check varieties ‘H5608’ and ‘N6428’ ‘H2012’ ‘H5608’ ‘N6428’Serum viscosity (centistokes) 9.2 9.6 7.6 Juice Bostwick (cm) 11.3 10.612.6 Soluble solids (° Brix) 5.3 5.0 5.1

Average of 2 years of trials in California in a total of 24 locations.

TABLE 3 Phenology ‘H2012’ ‘H5608’ ‘N6428’ Fruiting Season Short ShortShort Relative Maturity Medium Medium Long

TABLE 4 Adaptation ‘H2012’ ‘H5608’ ‘N6428’ Culture Field Field FieldPrinciple use Whole-pack, Whole-pack, Concentrate ConcentrateConcentrate Machine harvest Yes Yes Yes Regions of adaptabilityCalifornia Sacramento/ Yes - 2 Yes - 1 Yes - 2 upper SJ valleyCalifornia lower SJ valley Yes - 1 Yes - 2 Yes - 1 Northeastern USA NoNo n/tIf more than one category applies, they are listed in rank order.

Comparison of ‘H2012’ to Closest Varieties

Data in Table 1 is based primarily upon trials conducted inCollegeville, Calif. from two replications, non-staked, in a researchplot environment among two checks, ‘H5608’ and ‘H3402’. Data in Tables2, 3, and 4 are based upon observations and laboratory fruit qualitymeasurements made in numerous trials throughout California over a 2-yearperiod, relative to ‘H5608’ and a Nunhems variety ‘N6428’. Diseaseresistance and adaptability assessments are based upon DNA markersassociated with the disease resistance when available or numerousobservations collected in regions/climates with specific diseasepressure, specifically for ripe fruit rots, bacterial spot, bacterialcanker, early blight, and late blight, including Ontario, Canada.

Several characteristics can distinguish ‘H2012’ from the similarcommercial variety ‘H5608’, particularly when compared side-by-side intrials. The plant structure of ‘H2012’ is often slightly more compactthan ‘H5608’ with slightly more leaf roll; ‘H2012’ can be slightly laterto mature than ‘H5608’ but is often comparable. Horticulturally, the twomost prominent distinctions between ‘H2012’ and ‘H5608’ are theresistance to fusarium wilt race 3 and significantly improved internalfruit color of ‘H2012’. From a processing standpoint, ‘H2012’ hasslightly higher soluble solids but similar juice Bostick and serumviscosity to ‘H5608’. The higher soluble solids is of particular valuefrom a processing standpoint when producing commercial tomato paste.Both ‘H2012’ and the BASF/Nunhems company variety ‘N6428’ areCalifornia-adapted processing varieties with very good yield andresistance to fusarium wilt race 3. Distinguishing features betweenthese varieties include a higher viscosity of processed juice, asignificantly improved internal fruit color, and an earlier croppingperiod of ‘H2012’. These differences can be of significant economicvalue to processors.

Further Embodiments

Additional methods provided herein include, without limitation, chasingselfs. Chasing selfs involves identifying inbred plants among tomatoplants that have been grown from hybrid tomato seed. Once the seed isplanted, the inbred plants may be identified and selected due to theirdecreased vigor relative to the hybrid plants that grow from the hybridseed. By locating the inbred plants, isolating them from the rest of theplants, and self-pollinating them (i.e., “chasing selfs”), a breeder canobtain an inbred line that is identical to an inbred parent used toproduce the hybrid. Accordingly, another aspect of the disclosurerelates a method for producing an inbred tomato variety by: plantingseed of the tomato variety ‘H2012’; growing plants from the seed;identifying one or more inbred tomato plants; controlling pollination ina manner which preserves homozygosity of the one or more inbred plants;and harvesting resultant seed from the one or more inbred plants. Thestep of identifying the one or more inbred tomato plants may furtherinclude identifying plants with decreased vigor, i.e., plants thatappear less robust than plants of the tomato variety ‘H2012’. Tomatoplants capable of expressing substantially all of the physiological andmorphological characteristics of the parental inbred lines of tomatovariety ‘H2012’ include tomato plants obtained by chasing selfs fromseed of tomato variety ‘H2012’.

One of ordinary skill in the art will recognize that once a breeder hasobtained inbred tomato plants by chasing selfs from seed of tomatovariety ‘H2012’, the breeder can then produce new inbred plants such asby sib-pollinating, or by crossing one of the identified inbred tomatoplant with a plant of the tomato variety ‘H2012’.

The disclosure further includes introducing one or more desired traitsinto the tomato variety ‘H2012’. For example, the desired trait mayinclude male sterility, male fertility, herbicide resistance, insectresistance, disease resistance, and drought resistance.

The desired trait may be found on a single gene or combination of genes.The desired trait may be a genetic locus that is a dominant or recessiveallele. The genetic locus may be a naturally occurring tomato geneintroduced into the genome of a parent of the variety by backcrossing, anatural or induced mutation, or a transgene introduced through genetictransformation techniques. For a genetic locus introduced throughtransformation, the genetic locus may comprise one or more transgenesintegrated at a single chromosomal location. Accordingly, the disclosureprovides tomato plants or parts thereof that have been transformed withone or more transgenes (i.e., a genetic locus comprising a sequenceintroduced into the genome of a tomato plant by transformation) toprovide a desired trait. In one aspect, the one or more transgenes areoperably linked to at least one regulatory element. In one aspect, thetomato plant or plant part comprising a transgene has all thephysiological and morphological characteristics of tomato variety‘H2012’.

The gene(s) may be introduced to tomato variety ‘H2012’ through avariety of well-known techniques, including for example, molecularbiological, other genetic engineering, or plant breeding techniques,such as recurrent selection, backcrossing, pedigree breeding, molecularmarker (Isozyme Electrophoresis, Restriction Fragment LengthPolymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs),Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Amplified Fragment Length Polymorphisms (AFLPs), and Simple SequenceRepeats (SSRs) (also referred to as Microsatellites)), enhancedselection, genetic marker enhanced selection, and transformation.Accordingly, tomato seed, plants, and parts thereof produced by suchgenetic engineering or plant breed techniques are also part of thepresent disclosure.

Also provided herein are single locus converted plants and seedsdeveloped by backcrossing wherein essentially all of the morphologicaland physiological characteristics of an inbred are recovered in additionto the characteristics conferred by the single locus transferred intothe inbred via the backcrossing technique. A single locus may compriseone gene, or in the case of transgenic plants, one or more transgenesintegrated into the host genome at a single site (locus). One or morelocus conversion traits may be introduced into a single tomato variety.In one aspect, the tomato plant or tomato plant part comprising a singlelocus conversion has all the physiological and morphologicalcharacteristics of tomato variety ‘H2012’.

Deposit Information

A deposit of the tomato variety ‘H2012’ is maintained by HeinzSeedCompany, having an address at 6755 CE Dixon St, Stockton, Calif. 95206,United States of America. Access to this deposit will be availableduring the pendency of this application to persons determined by theCommissioner of Patents and Trademarks to be entitled thereto under 37C.F.R. § 1.14 and 35 U.S.C. § 122.

At least 625 seeds of tomato variety ‘H2012’ were deposited on Dec. 18,2020 according to the Budapest Treaty in the American Type CultureCollection (ATCC), P.O. Box 1549, MANASSAS, Va. 20108 USA. The deposithas been assigned ATCC number PTA-126914. Access to this deposit will beavailable during the pendency of this application to persons determinedby the Commissioner of Patents and Trademarks to be entitled theretounder 37 C.F.R. § 1.14 and 35 U.S.C. § 122. Upon allowance of any claimsin this application, all restrictions on the availability to the publicof the variety will be irrevocably removed for the enforceable life ofthe patent.

The deposits will be maintained in the ATCC depository, which is apublic depository, for a period of 30 years, or 5 years after the mostrecent request, or for the effective life of the patent, whichever islonger, and will be replaced if a deposit becomes nonviable during thatperiod.

1. Tomato seed designated as ‘H2012’, representative sample of seedhaving been deposited under ATCC Accession Number PTA-126914.
 2. A plantproduced by growing the seed of claim
 1. 3. A plant part from the plantof claim 2, wherein the plant part comprises at least one cell fromtomato variety ‘H2012’.
 4. The plant part of claim 3, wherein the partcomprises one or more of leaf, ovule, pollen, rootstock, scion, fruit,cotyledon, meristem, anther, root, root tip, pistil, flower, stem,calli, stalk, hypocotyl, pericarp, and portion thereof containing atleast one cell from tomato variety ‘H2012’.
 5. A tomato plant having allthe physiological and morphological characteristics of the tomato plantof claim
 2. 6. A plant part from the plant of claim 5, wherein the plantpart comprises at least one cell from tomato variety ‘H2012’.
 7. Theplant part of claim 6, wherein the part comprises one or more of leaf,ovule, pollen, rootstock, scion, fruit, cotyledon, meristem, anther,root, root tip, pistil, flower, stem, calli, stalk, hypocotyl, pericarp,and portion thereof containing at least one cell from tomato variety‘H2012’. 8-10. (canceled)
 11. Pollen of the plant of claim
 2. 12. Anovule of the plant of claim
 2. 13. A tissue culture of regenerable cellsfrom the plant part of claim 3, wherein said tissue culture ofregenerable cells has all the physiological and morphologicalcharacteristics of tomato variety ‘H2012’.
 14. A tomato plantregenerated from the tissue culture of claim 13, the plant having all ofthe physiological and morphological characteristics of tomato variety‘H2012’, wherein a representative sample of seed has been depositedunder ATCC Accession Number PTA-126914.
 15. A protoplast produced fromthe tissue culture of claim 13, wherein a plant regenerated from theprotoplast has all of the physiological and morphologicalcharacteristics of tomato variety ‘H2012’.
 16. A method of producing atomato plant derived from tomato variety ‘H2012’, the method comprisingcrossing the plant of claim 2 with another tomato plant to produce an F₁hybrid tomato plant.
 17. The method of claim 16, further comprisingharvesting seed from the F₁ hybrid tomato plant.
 18. The method of claim16, further comprising crossing the F₁ hybrid tomato plant with itselfor another plant to produce seed from a progeny plant.
 19. The plant ofclaim 2, said plant having all the physiological and morphologicalcharacteristics of tomato variety ‘H2012’, and further comprising atransgene.
 20. The plant of claim 2, said plant having all thephysiological and morphological characteristics of tomato variety‘H2012’, and further comprising a single locus conversion.
 21. A methodfor producing a tomato fruit, the method comprising: growing the tomatoplant of claim 2 to produce a tomato fruit; and harvesting the tomatofruit.
 22. A method for producing a tomato seed comprising:self-pollinating the tomato plant of claim 2; and harvesting theresultant tomato seed.
 23. A method of vegetatively propagating theplant of claim 2, the method comprising: obtaining a part of the plant;and regenerating a plant from the part, the regenerated plant having allof the physiological and morphological characteristics of tomato variety‘H2012’.